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AURKA showed results similar to those of both immunoblotting (Supplementary Fig. Immunofluorescence staining (Supplementary Fig. The cytoplasmic AURKA level was lower in breast cancer cells compared with the non- transformed MCF- 1. Consistent with this, a similar expression pattern of nuclear AURKA was observed with immunohistochemistry (IHC) staining (Fig. In contrast, AURKA was also highly expressed in the nuclear fraction of breast cancer tissue. We first examined cytoplasmic and nuclear expression of AURKA in breast cancer and adjacent normal tissues (Fig.ĪURKA was detected in the cytoplasm in normal breast tissue. Nuclear AURKA enhances breast CSC phenotype. These findings identify a previously unknown oncogenic property of the spatially deregulated AURKA in tumorigenesis and provide a potential therapeutic opportunity to overcome kinase inhibitor resistance. RNP K) in the nucleus and acts as a transcription factor in a complex that induces a shift in MYC promoter usage and activates the MYC promoter.īlocking AURKA nuclear localization inhibits this newly discovered transactivating function of AURKA, sensitizing resistant BCSC to kinase inhibition. Instead, AURKA preferentially interacts with heterogeneous nuclear ribonucleoprotein K (hn. Unexpectedly, this function is independent of its kinase activity. Here we show that AURKA translocates to the nucleus and causes distinct oncogenic properties in malignant cells by enhancing breast cancer stem cell (BCSC) phenotype. Centrosome- localized mitotic Aurora kinase A (AURKA) facilitates G2/M events. Nuclear AURKA acquires kinase- independent transactivating function to enhance breast cancer stem cell phenotype. The fast growing developments in machinery industry have led to the need for designing light-weight and more reliable components. Because of its compact structure, high transmission precision and strong load bearing capacity, gear transmission is widely used in vehicles, machine tools, railways, aerospace and other mechanical systems. Gears are the most important components in machinery for transferring power and motion. The prediction of crack propagation path shows the tooth fracture in our case study and is consistent with the experimental results in the literature. The variation of meshing load results in longer fatigue life prediction compared to conventional non-coupling analysis. The load carried by the cracked tooth during the double-tooth engagement is found to decrease with crack growth. The existence of tooth root crack causes the reduction of meshing stiffness and the variation of contact load on cracked tooth. The time-varying moving load obtained from meshing analysis was then applied as the boundary condition of crack propagation. The crack morphology in the meshing analysis was updated by extracting the signed distance function value from crack propagation analysis. A standard and an extended finite element model were developed for gear pair meshing and crack propagation, respectively. This paper presents a study of the coupling behavior of spur gear meshing and tooth root crack propagation. Fatigue and fracture behavior at tooth root during gear meshing are critical to the gear transmission performance. When he told his friends about his “relationship”, he was met by backslapping and hearty congratulations-if he had been a girl and she a man, it would’ve been seen as the child sexual abuse (CSA) by a predator that it was. This extends to pubescent boys abused by older women, as a relative of mine was by his teacher. Another fallout of the female-as-weak, male-as-strong binary is that women aren’t seen as sexual initiators and potential perpetrators of abuse boys aren’t seen as needing protection and care. 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Jenisnya dibagi menjadi dua jenis varietas yaitu kacang almond yang memiliki rasa manis dan kacang almond yang mempunyai rasa pahit. Untuk anda yang belum mengetahui tentang kacang almond, kacang ini adalah kacang super yang di dalamnya memiliki banyak nutrisi yang baik untuk tubuh. Ini dikarenakan daya beli masyarakat pada kelas menengah ke bawah tergolong rendah, mengingat harga kacang almond yang relatif lebih mahal jika dibandingkan dengan jenis kacang yang lainnya. Tetapi ada juga sebagian orang yang belum mengetahui mengenai manfaat dari kacang almond apalagi untuk masyarakat yang menengah ke bawah. Maka dari itu banyak sekali orang yang rajin mengonsumsi kacang untuk menjaga kecantikan dan kesehatannya. Karena produk olahannya dapat dipakai untuk menambah kecantikan dan meningkatkan kesehatan. Tidak tanggung – tanggung, kandungan yang ada di dalamnya sangat bermanfaat untuk para wanita. Dari segi ukuran, kacang almond juga mempunyai bentuk yang lebih besar daripada kacang tanah atau jenis kacang lainnya. Kacang almond adalah kacang yang memiliki kandungan nutrisi dan vitamin yang tinggi. Tentu saja anda sudah tidak asing lagi dengan jenis kacang satu ini. Untuk Pemesanan Silahkan Hubungi: WhatsApp/SMS 085735800382 Apa yang anda pikirkan ketika mendengar kata kacang almond? |
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